Heating Greatly Speeds Coomassie Blue Staining and Destaining

Heating greatly speeds Coomassie blue staining and destaining.

All loading buffers allowed the target amplification at 0.25 and 0.5 ×final concentration (Figure 2). However, at 0.5 × final concentration , there was a decrease in the amplification that was more pronounced than that observed with the 359 bp target depicted in Figure 1. After the 0.25 ×gel-loading buffers in the PCR mixture proved to be feasible even when the bacterial lysate was diluted 1000...

Rapid Coomassie blue staining and destaining of polyacrylamide gels.

Detergent-mediated destaining of coomassie brilliant blue-stained SDS polyacrylamide gels.

A simple and one-step detergent-mediated destaining procedure for SDS Polyacrylamide gels for proteins is described. Suspension (5%, w/v) of a commercially available household detergent, Vim Ultra, has been found to be very efficient in destaining polyacrylamide gels without interfering with the resolution of proteins. As compared to the routinely used solvent (methanol-acetic acid-water)-media...

Environmentally benign staining procedure for electrophoresis gels using Coomassie Brilliant Blue.

Dimethylformamide interferes with Coomassie dye staining of proteins on blue native gel electrophoresis.

Blue native gel electrophoresis (BN-PAGE) is used extensively for characterization of mitochondrial respiratory complexes and uses the binding of Coomassie brilliant blue G-250 to visualize proteins. Oxidative modification of sulfhydryl groups of such proteins can be evaluated by labeling with iodoacetamide conjugated to biotin (BIAM) and detected with streptavidin peroxidase on Western blots f...